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Confocal Laser Scanning Microscope

Olympus FV3000RS (Evident, Olympus life science) utilizes two high sensitivity spectral detectors and one spectral detector and is capable of multichannel Truspectral imaging, enabling simultaneous imaging with flexibility and high sensitivity. Four lasers (405nm, 488nm, 561nm, 640nm) have offered the opportunity to select a vast variety of dyes and detectors. The microscope is equipped with hybrid scanner system, employing a resonant scanner and a galvanometer scanner, for capturing live phenomena with high speed and for recording complex structures with high resolution respectively. Zero drift compensator (ZDC-2) facilitates long term imaging. 2D, 3D and MATL images are acquired via XY, XYZ or XYT and can be mapped or stitched. Tilt adjustment in Z direction has made easier with the FV3000RS.  Lambda series can be acquired by a single channel or multiple channels. The system is ideal in performing colocalization, 2D deconvolution, FRAP and FRET analysis.

The guava easyCyte™ 12

Selected publications

  • Dayarathna, S., Jeewandara, C., & Malavige, G. N. (2020, December). Use of Confocal Microscopy to Characterise DENV Infection and NS1 Expression in C6/36 and HepG2 Cells in Utilising Them as Models to Screen for the Efficacy of Antiviral Drugs. In 2020 From Innovation to Impact (FITI) (Vol. 1, pp. 1-5). IEEE.

BD FACSAria™ III

The FACSARIA III is a high-speed fixed-alignment benchtop cell sorter which is highly sensitive and can handle high-throughput. This is equipped with 3 lasers (488, 561, 633) and can assay up to 13 colours. Cell sorting can be done to tubes or 96-well plates.

Selected publications

  • Jeewandara C, Guruge D, Pushpakumara PD, Kamaladasa A, Aberathna IS, Ramu ST, Gunasekera B, Wijesinghe A, Dissanayake O, Kuruppu H, Ranasinghe T, Jayathilaka D, Dayarathna S, Ekanayake D, Jayamali J, Gamalath N, Mudunkotiwa A, Somathilake G, Dissanayake M, Harvie M, Nimasha T, Madusanka D, Jayadas T, Wijayamuni R, Schimanski L, Rijal P, Tan TK, Townsend A, Ogg GS, Malavige GN. Immune Responses to a Single Dose of the AZD1222/Covishield Vaccine at 16 Weeks in Individuals in Sri Lanka. J Immunol 2021 Dec 1;207(11):2681-2687. doi: 10.4049/jimmunol.2100762. Epub 2021 Nov 8. PMID: 34750205.
  • Jayaratne, H.E., Wijeratne, D., Fernando, S., Kamaladasa, A., Gomes, L., Wijewickrama, A., Ogg, G.S. and Malavige, G.N. (2018), Regulatory T-cells in acute dengue viral infection. Immunology, 154: 89-97. https://doi.org/10.1111/imm.12863
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