ELISA and Elispot

Cobas e 411 analyzer

The cobas e 411 analyzer is a fully automated analyzer which uses Electrochemiluminescence (ECL) technology for immune assays. Our analyzer has a disk sample handling system for both quantitative and qualitative in vitro assay determinations for a broad range of applications.

External uses

ECL technology has a wide range of applications in quantitative and qualitative in vitro assay determinations including anemia; bone, cardiac and tumor markers; critical care; fertility/hormones; maternal care; and infectious diseases.

Selected Publications

Kinetics of immune responses to the AZD1222/Covishield vaccine with varying dose intervals in Sri Lankan individuals. Jeewandara C, Aberathna IS, Gomes L, Pushpakumara PD, Danasekara S, Guruge D, Ranasinghe T, Gunasekera B, Kamaladasa A, Kuruppu H, Somathilake G, Dissanayake O, Gamalath N, Ekanayake D, Jayamali J, Jayathilaka D, Mudunkotuwa A, Harvie M, Nimasha T, Wijayamuni R, Schimanski L, Rijal P, Tan TK, Dong T, Townsend A, Ogg GS, Malavige GN. Jeewandara C, et al. Immun Inflamm Dis. 2022 Apr;10(4):e592. doi: 10.1002/iid3.592. Immun Inflamm Dis. 2022. PMID: 35349749 Free PMC article.

ELLA

ELLA (version 3.4.0.52, Protein Simple, USA) is a fully automated immunoassay instrument that allows fast performance of ELISA assays without any manual steps. This system uses cartridges that allow the measurements of biomarkers in different types of samples. The cartridges use microfluidics based technology that enables the measurements of single analytes, multiple analytes and also multiplexed analytes. The results obtained using the ELLA is highly sensitive and reproducible. This system allows the researcher to perform an automated immunoassay and automatically process the data acquired. It also allows the researcher to view, analyse, and extract and save fluorescence and concentration data to be used for further analysis.

Selected Publications

Dayarathna, S., Jeewandara, C., Gomes, L. et al. Similarities and differences between the ‘cytokine storms’ in acute dengue and COVID-19. Sci Rep 10, 19839 (2020). https://doi.org/10.1038/s41598-020-76836-2

ELISA Plate Reader: MPSCREEN MR-96A

ELISA is an abbreviation for “Enzyme Linked Immunosorbent Assay” and is a common laboratory technique. We use this technique to measure the analyte’s (antibodies or antigens most likely) concentration in solutions. The wavelength of the coloured end product is the indicator of the amount of analytes in the original sample which is then measure via the ELISA plate reader.

AID iSpot (ELR08IFL)

The basic 96- and 384-well plate EliSpot & FluoroSpot Reader

The AID iSpot is one of the most successful EliSpot/FluoroSpot Reader developments in recent years. The AID iSpot for the first time allows analyzing both: Enzymatic and fluorescent (FluoroSpot) based EliSpot assays. The AID iSpot comprises the same outstanding functionality, such as layout-generator, rule-compiler, various export possibilities etc., as the AID Classic. Beside all of the popular functions of the AID Classic, the AID iSpot simultaneously allows for 1-, 2- or even 3-color FluoroSpot analysis. A simple “one-click switch” between enzymatic and fluorescent mode, without the need of hardware changes, is enough to switch from one mode to the other.

Selected Publications

C.Jeewandara et al., “Immune responses following the first dose of the Sputnik V (Gam-COVID-Vac),” Sci. Reports 2022 121, vol. 12, no. 1, pp. 1–10, Feb. 2022, doi: 10.1038/s41598-022-05788-6.
P. D. Pushpakumara et al., “Identification of Immune Responses to Japanese Encephalitis Virus Specific T Cell Epitopes,” Front. Public Heal., vol. 8, Feb. 2020, doi: 10.3389/fpubh.2020.00019.
C. Jeewandara et al., “Persistence of immune responses to the Sinopharm/BBIBP-CorV vaccine,” Immunity, Inflamm. Dis., vol. 10, no. 6, p. e621, Jun. 2022, doi: 10.1002/IID3.621.
C. Jeewandara et al., “Immune responses to a single dose of the AZD1222/Covishield vaccine in health care workers,” Nat. Commun., vol. 12, no. 1, Dec. 2021, doi: 10.1038/s41467-021-24579-7.
C. Jeewandara et al., “Immune responses to Sinopharm/BBIBP-CorV in individuals in Sri Lanka,” Immunology, vol. 167, no. 2, pp. 275–285, Oct. 2022, doi: 10.1111/IMM.13536.
P. D. Pushpakumara et al., “Development and validation of an assay for detection of Japanese encephalitis virus specific antibody responses,” PLoS One, vol. 15, no. 10, p. e0238609, Oct. 2020, doi: 10.1371/journal.pone.0238609.
C. Jeewandara et al., “Seroprevalence of SARS-CoV-2 Infection in the Colombo Municipality Region, Sri Lanka,” Front. public Heal., vol. 9, Nov. 2021, doi: 10.3389/FPUBH.2021.724398.